Morfologia przedniej torebki soczewki po kapsuloreksji
Cataract Lens Anterior Capsule Morphology after Capsulorhexis Procedure
Piotr Skopiński1,2, Ewa Langwińska-Wośko1, Zbigniew Kiciak1, Magdalena Korwin-Rujna1
1Katedra i Klinika Okulistyki II Wydziału Lekarskiego
Akademii Medycznej w Warszawie
Kierownik: prof. dr hab. n. med. Jerzy Szaflik
Samodzielny Publiczny Kliniczny Szpital Okulistyczny w Warszawie
2Zakład Histologii i Embriologii Instytutu Biostruktury Akademii Medycznej w Warszawie
Kierownik: prof. dr hab. n. med. Stanisław Moskalewski
Summary: The paper describes
microscopic and submicroscopic structure of the cataract
lens anterior capsule obtained during capsulorexis
procedure. Thickness and spatial architecture of the
lenticular collagen fibers according to the patient’s
age, cataract type and general diseases were evaluated.
Purpose: The purpose of the study was evaluation of thickness and spatial architecture of the lenticular collagen fibers according to the patient’s age, cataract type and general diseases, especially diabetes mellitus.
Material and Methods: The paper describes microscopic and submicroscopic structure of the cataract lens anterior capsule obtained during capsulorhexis procedure performed in the Department of Ophthalmology Medical University of Warsaw. 36 cataract lens anterior capsules were obtained during capsulorhexis procedure. Anterior capsules were studied in light and transmission electron microscope after fixation in glutaraldehyde, lavaging in phosphate buffer solution, dehydration in acetone and ethanol solutions and placing in epoxide resin.
Results: Light microscope: In the studied capsules, in all cases, single-layer cuboidal lenticular epithelium underlying homogenous lenticular capsule was observed. Differences in epithelium and capsule morphology in cases associated with systemic diseases especially diabetes mellitus and the control were not observed. Electron microscope: Following epithelium cells morphology changes were observed: mitochondria vacuolization and swelling, numerous vacuoles in the peribasal cells part on the margin with lens capsule, single epithelium cells necrosis. Lens capsules are made of homogenous collagen membrane, which does not differ in individual cases.
Conclusions: In studied cases anterior capsule thickness was directly proportional to patients age. Some systemic diseases incidence may influence on an anterior capsule thickness (capsule is thinner in patients with systemic, especially metabolic diseases). Relationship between lenticular capsule thickness and gender was not stated.
Keywords: cataract, capsulorexis, anterior capsule thickness, transmission electron microscope, light microscope.